重庆医学2011，Vol.40Issue(34)：3482-3483,2.DOI:10.3969/j.issn.1671-8348.2011.34.020
酶消化法分离老龄SD大鼠血管平滑肌细胞
A method of enzyme digestion for dissociation of vascular smooth muscle cells in aged SD rats
王明勇 1陈枫 1陈庄1
作者信息
- 1. 泸州医学院附属医院医学实验中心,四川泸州,646000
- 折叠
摘要
Abstract
Objective To culture vascular smooth muscle cells of aged Sprague Dawley(SD) rats using enzymatic dissociation method and provide a large number of primary smooth muscle cells for research of vascular diseases. Methods Aortas of aged SD rats acquired sterilely were subjected to digestion. Vascular smooth muscle cells were digested and dissociated by 0. 2% Ⅱ collage-nase,purified by natural purification and differential adhesion, α actin of smooth muscle cells were identified by immunohistochemis-try. Results Immunohistochemical staining demonstrated the cell purity was more than 95%. Conclusion Enzymatic digestion method employed in vascular smooth muscle cell culture is conducive to obtaining a large number of cells with high purity for experimental research.关键词
细胞培养技术/肌,平滑,血管/免疫组织化学/肌动蛋白类/大鼠Key words
cell culture techniques/muscle,smooth,vascular/immunohistochemistry/actins/rats引用本文复制引用
王明勇,陈枫,陈庄..酶消化法分离老龄SD大鼠血管平滑肌细胞[J].重庆医学,2011,40(34):3482-3483,2.
