浙江医学2011，Vol.33Issue(11)：1593-1595,3.
外源性VEGF和bFGF基因治疗大鼠脑缺血疗效的比较研究
VEGF and bFGF gene therapy for cerebral ischemia in rats
摘要
Abstract
Objective To evaluate VEGF and bFGF gene therapy for cerebral ischemia in rats. Methods The cerebral ischemia model was established by permanent occlusion of middle cerebral artery(MCAO) with nylon suture embolization and cer-clage in rats. The recombinant adeno- associated virus mediated vascular endothelial growth factor gene (rAAV- VEGF) and/or recombinant adeno- associated virus mediated basic fibroblast growth factor(rAAV- bFGF) were injected through the lateral cerebral ventricle within 24h after MCAO. On the dl4 after the rAAV- VEGF and rAAV- bFGF injection,the rats were sacrificed and brain samples were taken for study. Results From observing the positive cells stained by terminal- deoxynucleotidyl transferase mediated nick end labeling (TUNEL) in the ischemic penumbra and analyzing the size of infarct by TTC staining , it was found that a significant reduction in the ratio ofapoptosis cells and infarct volume was observed in the therapeutic groups when compared to the MCAO and sham- operated group(P<0.05), and it was more prominent in the therapeutic group by two genes combined therapy than by rAAV- VEGF or rAAV- bFGF alone(P<0.05). Conclusion Our results indicate that the VEGF together with bFGF gene therapy of the ischemic cerebrovascular diseases is a safe and effective gene transferring approach that may be suitable for clinical application.关键词
脑缺血/血管内皮细胞生长因子/碱性成纤维细胞生长因子/脑室途径/基因治疗/大鼠Key words
Brain ischemia/Vascular endothelial growth factor/Basic fibroblast growth factor/Intra- ventricular/Gene therapy/Rats引用本文复制引用
邵林华,孙青芳,卞留贯,陈亦华,程振宇,郑龙,俞建华,张晨冉,顾吕伟..外源性VEGF和bFGF基因治疗大鼠脑缺血疗效的比较研究[J].浙江医学,2011,33(11):1593-1595,3.基金项目
浙江省卫生厅科研计划资助项目(2007B221) （2007B221）
金华市科技局科研计划资助项目(2007-03-044) （2007-03-044）
