西南国防医药2011，Vol.21Issue(12)：1297-1299,3.DOI:10.3969/j.issn.1004-0188.2011.12.007
肾移植慢性排斥反应动物模型的建立与质量控制
Animal model of renal transplantation chronic rejection:establishment and quality control
李健 1许亚宏 1马小平 1谭娟 1张艮甫 2黄赤兵 2陈萍 1郭瑜1
作者信息
- 1. 610021,成都,解放军452医院泌尿外科
- 2. 第三军医大学附属新桥医院泌尿外科
- 折叠
摘要
Abstract
Objective To build up a stable and repeatable animal model of renal transplantation chronic rejection. Methods The F344 - Lewis I - line male rats were used as donor and recipient. The two kidneys of one donor were respectively transplanted into two recipients. The end - to - end anastomosis was performed in the transplantation of renal artery and vein and the restitution of urin urinary tract. In the operation, the left kidney of the recipient was excised for implantation of the transplanted kidney, and the right kidney was remained. Three days after the operation, the vessels of the right kidney were ligated in vitro. Results Forty cases received the allograft renal transplantation. The operation time was ( 90 ± 27 ) min. The time of kidney excision was ( 25 ± 7 ) min. The transplantation time was( 37 ± 17 )min. The warm and the cold ischemia time of transplanted kidneys was( 20 ± 5 )s and( 37 ± 3 )min, respectively. The kidney excision in 20 cases and the transplantation in 40 cases were all successful. Thirty five chronic rejection models were obtained. The survival time of the rats receiving transplantation was( 128 ± 34 ) d. The achievement ratio was 87. 5%. The pathological specimens of mild and typical renal transplantation chronic rejection were respectively obtained 10 w and 14 w after the operation. Conclusion The key factors of successful establishment of chronic rejection animal model include correct selection of animals, proficiency in microsurgical techniques, reduction of both warm and cold ischemic time and reasonable immunosuppressive therapies.关键词
肾移植/慢性排斥反应/动物模型/质量控制Key words
kidney transplantation/ chronic rejection/ animal model/ quality control分类
医药卫生引用本文复制引用
李健,许亚宏,马小平,谭娟,张艮甫,黄赤兵,陈萍,郭瑜..肾移植慢性排斥反应动物模型的建立与质量控制[J].西南国防医药,2011,21(12):1297-1299,3.
