武警医学2011，Vol.22Issue(12)：1052-1055,4.
赖氨酸对甲基苯丙胺神经毒性的保护作用
Protection of lysine against methamphetamine-induced neurotoxicity
范利斌 1李仲影 1康剑锋 1吴纯 1范丽 1李杏丽 1曹晓凯1
作者信息
摘要
Abstract
Objective To investigate the protective effect of lysine against methamphetamine - induced neurotoxicity in C6 cells. Methods C6 cells were randomly divided into control group, methamphetamine(2 mM, 3 mM, 4mM) group, lysine group, methamphetamine + lysine group. To determine the oxidative damage induced by methamphetamine, catalase ( CAT) and glutathione peroxidase (GPx) were detected in C6 cells. To estimate apoptosis, flow cytometry analysis using annexin V - FITC and propidium iodide staining as well as detection for caspase - 3 and Bel - 2 proteins were performed after methamphetamine exposure for 48 hours. Results Lysine reversed the change of the form of C6 cells caused by methamphetamine and greatly improved CAT and GPx activities. It also reversed the expression of Bel - 2 apoptosis protein induced by methamphetamine. Conclusions Lysine can ameliorate methamphetamine - induced oxidative insult and apoptosis in C6 cells, probably due to its antioxidant activity rather than the activation of GAB A receptors.关键词
甲基苯丙胺/赖氨酸/C6细胞/氧化应激/凋亡/γ-氨基丁酸Key words
methamphetamine/ lysine/ C6 cell/ oxidative stress/ apoptosis/ GABA receptors分类
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范利斌,李仲影,康剑锋,吴纯,范丽,李杏丽,曹晓凯..赖氨酸对甲基苯丙胺神经毒性的保护作用[J].武警医学,2011,22(12):1052-1055,4.
