大鼠肝缺血再灌注后肝细胞内糖原含量及细胞膜ATP酶活力的变化OACSTPCD
Changes of hepatocellular glycogen content and activities of membran enzyme after liver ischemia reperfusion in rats
目的 探讨肝细胞内糖原含量及细胞膜ATP酶活力与肝缺血再灌注(I/R)损伤的关系及相关机制.方法 健康雄性SD大鼠16只,随机分为2组(每组8只),①对照组:术中只分离肝周围韧带,不作肝门阻断及再灌注;②I/R组:进行45 min的部分肝门阻断及60 min的再灌注.取下腔静脉血2 mL,并迅速切取缺血肝组织.检测血清丙氨酸转氨酶(ALT)、门冬氨酸转氨酶(AST)、乳酸脱氢酶(LDH);测定肝组织中超氧化物歧化酶(SOD)、丙二七醛(MDA)、…查看全部>>
Objective To investigate the relationship between hepatocellular glycogen content as well as activities of membrane ATPase and liver ischemia reperfusion (I/R) injury in rats. Methods Sixteen healthy male SD rats were randomly divided into 2 groups. Control group was served as sham operated control. I/R group 2 was subjected to 45 min lobar, rather than totlal hepatic ischemia and 60 min reperfusion. Blood samples (2 mL) were drawn from inferior vena cava fo…查看全部>>
侍阳;宋庆伟;李向农;周晓俊;钱海鑫
徐州医学院附属医院,普外科,江苏,徐州,221002徐州医学院附属医院,普外科,江苏,徐州,221002徐州医学院附属医院,普外科,江苏,徐州,221002苏州大学第一附属医院,普外科,江苏,苏州,215006苏州大学第一附属医院,普外科,江苏,苏州,215006
医药卫生
肝脏再灌注损伤肝糖原ATP酶大鼠
liverreperfusion injury liver glycogen ATPase rats
《肝胆胰外科杂志》 2011 (6)
478-480,3
江苏省自然科学基金项目(BK2007057).
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