肝胆胰外科杂志2011，Vol.23Issue(6)：484-486,3.
岩藻糖在正常人肝细胞、低转移人肝癌细胞和高转移人肝癌细胞表面的含量变化
The variation of fucose on the surfuace of normal hepatocytes,low metastatic hepatocellular carcinoma cells and high metastasis hepatoceHular carcinoma cells
廖晖 1廖彩仙 1王宇 1龚祖元 1胡育海1
作者信息
- 1. 南方医科大学附属南方医院,肝胆外科,广东,广州,510515
- 折叠
摘要
Abstract
Objective To compare the differential expression level of fucose on the surface of normal hepa-tocytes, low metastatic hepatocellular carcinoma cells and high metastasis hepatocellular carcinoma cells. Methods The amounts of fucose expressed on normal hepatic cells, low metastatic hepatocellular carcinoma cells and high metastasis hepatocellular carcinoma cells were detected by flow cytometry using fucose-specific fluorescein isothiocyanate-ulex europaeus agglutinin (FITC-UEA) in single cell suspensions. Results The fluorescence intensity detected in hepatocytes was lower than those of low metastatic hepatocellular carcinoma cells and high metastasis hepatocellular carcinoma cells (P<0.05 for both comparisons). Compare with low metastatic hepatocellular carcinoma cells, the fluorescence intensity of high metastasis hepatocellular carcinoma cells was greater (P<0.05). Conclusion The amounts of fucose on cell surface was found to be closely related to the development and metastasis of hepatocellular carcinoma cells. After cell canceration, the fucose content on cell surface increases, and the higher the metastatic potential of cancerous cells, the higher the content of fucose.关键词
肝细胞/肝癌细胞/岩藻糖/荆豆凝集素Key words
hepatocyte/ hepatocellular carcinoma cell/ fucose/ ulex europaeus agglutinin分类
医药卫生引用本文复制引用
廖晖,廖彩仙,王宇,龚祖元,胡育海..岩藻糖在正常人肝细胞、低转移人肝癌细胞和高转移人肝癌细胞表面的含量变化[J].肝胆胰外科杂志,2011,23(6):484-486,3.
