重庆医学2011，Vol.40Issue(35)：3594-3596,封3,4.DOI:10.3969/j.issn.1671-8348.2011.35.027
shRNA靶向干扰PRDXⅢ对人胶质瘤细胞株U251的影响
Effects of shRNA interference targeting PRDXⅢ on U251 human glioma cells
焦庆芳 1李松 1刘展 1游潮 2王春婷3
作者信息
- 1. 河南省郑州人民医院神经外科,450003
- 2. 四川大学华西医院神经外科,成都,610041
- 3. 四川大学生物治疗国家重点实验室,成都,610041
- 折叠
摘要
Abstract
Objective To investigate the effects of Peroxiredoxins Ⅲ (PRDX Ⅲ ) gene on proliferation and apoptosis of U251 human glioma cells in vitro. Methods U251 cells were divided into blank control group, empty vector transfection group, unrelated sequence transfection group and interference group. PRDX Ⅲ interference fragment was transfected into U251 glioma cells via lipo-some. RT-PCR and Western blot were employed to detect the mRNA and protein expression of PRDX Ⅲ in transfected cells, respectively. Flow cytometry(FCM) ,DNA ladder and propidium iodide (PI) staining were used to determine the apoptosis of transfected U251 cells. MTT was adopted to measure the rate of growth inhibition of transfected U251 cells. Results In interference group,the mRNA and protein expression of PRDX Ⅲ of cells were reduced significantly after transfection, with inhibitory rates of 76. 0% and 63. 9% respectively,DNA ladder showed obvious ladder-shaped electrophoretic bands,and PI staining revealed nuclear chromatin with high staining and some nuclear fragments. Apoptotic rates of cells in blank control group,empty vector transfection group, unrelated sequence transfection group and interference group were (2.26 ± 0. 64)%,(2. 68 ± 0. 74)%,(3. 54 ± 0. 62)% and (35. 90 ± 4. 85) %, respectively. Apoptotic rate of cells in interference group was markedly higher than that in other groups(P = 0. 00). Growth inhibitory rate of U251 cells in interference group was 44. 40%. Conclusion PRDX HI targeted interference can significantly inhibits proliferation and increases apoptosis of U251 cells.关键词
神经胶质瘤/RNA,小分子干扰/细胞凋亡/过氧化物酶/转染Key words
glioma/ RNA, small interfering/ apoptosis/ peroxiredoxin/ transfection引用本文复制引用
焦庆芳,李松,刘展,游潮,王春婷..shRNA靶向干扰PRDXⅢ对人胶质瘤细胞株U251的影响[J].重庆医学,2011,40(35):3594-3596,封3,4.
