分析测试学报2011，Vol.30Issue(8)：892-896,5.DOI:10.3969/j.issn.1004-4957.2011.08.012
鸡组织中3种青霉素类药物残留检测的前处理方法比较研究
Comparison of Pretreatment Methods for th.e Simultaneous Determination of Three Kinds of Penicillin Residues in Chicken Tissues
摘要
Abstract
Different pretreatment methods (e. G. Acetonitrile extraction, acetonitrile extraction - solid phase extraction ( SPE ) , matrix solid phase dispersion ( MSPD) extraction and MSPD - SPE ) for the simultaneous analysis of penicillin, ampicillin and amoxicillin in chicken tissues were compared with HPCE. The results indicated that, among these methods, the MSPD method was of high precision, high recovery, easy operation and good practicability. Under the optimal chromatographic conditions, the calibration curves were linear in the range of 100-1 000 μg/L for penicillin and ampicillin , and 50-1 000 μg/L for amoxicillin. At the spiked concentrations of 50, 500, 1 000 μg/kg, the recoveries of these three drugs in chicken tissues ranged from 73% to 96% with relative standard deviations (RSDs) of 2. 9% -9. 8% . The intra-RSDs and inter-RSDs ranged from 4. 0% to 12.0% . The detection limits of penicillin, ampicillin and amoxicillin were 20, 30, 10 μg/kg, respectively, and their quantitation limits were 50, 50, 40 μg/kg, respectively. The method could meet the re quirements for the residue analysis in accuracy and precision.关键词
基质固相分散萃取/高效毛细管电泳/样品前处理/青霉素类药物/残留/鸡组织Key words
matrix solid phase dispersion extraction/ high performance capillary electrophoresis ( HPCE ) / sample pretreatment/ penicillins/ residues/ chicken tissues分类
化学化工引用本文复制引用
汪雪雁,祁克宗,陈玎玎,檀华蓉,薛秀恒..鸡组织中3种青霉素类药物残留检测的前处理方法比较研究[J].分析测试学报,2011,30(8):892-896,5.基金项目
国家十一五科技支撑项目子项目(2006 BAK02 A08-4) （2006 BAK02 A08-4）
