河南农业大学学报2011，Vol.45Issue(4)：391-394,4.
人工蛹虫草核苷类成分超声提取工艺优化HPLC定量分析
Ultrasonic extraction and analysis of nucleosides by HPLC in Cordyceps militaris
摘要
Abstract
This paper aims to optimize the ultrasonic extraction conditions and to develop a method by HPLC for simultaneously determining four nucleotides (uridine,guanosine,adenosine and cordyce-pin) in Cordyceps militaris. Optimization of the ultrasonic extraction conditions was conducted by orthogonal layout. The HPLC analysis working conditions are as follows; column was Amethyst C18-H (4.6 mm x250 mm,5μm) ; volume ratio of methanol-water was 15:85,retention time was 18 min; flowing rate was 0. 8mL·min-1; temperature is 35℃; detective wavelength was 260 nm; injection volume was 10μL. The optimum extraction conditions were confirmed as follows; the ratio of sample to water(m/V) was 1 = 30; solvent is pure water; extraction temperature was 25℃; extraction time was 45 min. The HPLC method used in this experiment was simple and convenient,hence it is valuable for quality evaluation of Cordyceps militaris.关键词
蛹虫草/核苷/高效液相色谱/超声提取Key words
Cordyceps militarist nucleoside/HPLC/ultrasonic extraction分类
生物学引用本文复制引用
申进文,孙晓萍,赵旭,阮元,张耀光,麻兵继..人工蛹虫草核苷类成分超声提取工艺优化HPLC定量分析[J].河南农业大学学报,2011,45(4):391-394,4.基金项目
河南省科技攻关计划项目(102102310186) （102102310186）
