分析化学2011，Vol.39Issue(9)：1373-1379,7.DOI:10.3724/SP.J.1096.2011.01373
亲水性相互作用色谱-四极杆串联线性离子阱质谱测定蛋白食品中L-羟脯氨酸残留
Determination of L-Hydroxyproline by Hydrophilic Interaction Chromatography Coupled Tandem Quadrupole Linear Ion Trap Mass Spectrometry in Protein Contained Food
摘要
Abstract
A method based on hydrophilic interaction chromatography-quadrupole/linear ion trap-mass spectrometry (HILIC-q/LTQ MS) for qualitative and quantitative determination of L-hydroxyproline (L-HYP) as target compound in protein contained-food was presented. L-hydroxyproline in samples were extracted with acetonitrile after enzymolysis, followed by a further cleanup using HLB solid phase extraction column to'remove lipid soluble interferes. The L-HYP residue was separated upon a hydrophilic interaction chromatography column and determined by quadrupole-linear ion trap-mass spectrometry. Information dependent acquisition scan function (IDA) combined with enhanced product ion scan (EPI) library was used to confirm the content of L-HYP, while quantification was operated by MRM mode. The recoveries ranged from 73. 4% to 114. 2% (n=6) and the RSD between 2. 5% and 14. 4% (n = 6) in 12 kinds of protein contained matrices respectively.关键词
L-羟脯氨酸/亲水性相互作用色谱/四极杆串联线性离子阱质谱/皮革水解蛋白Key words
L-Hydroxyproline/ Hydrophilic interaction chromatography/ Quadrupole-linear ion trap-mass spectrometry/ Protein contained food引用本文复制引用
赵善贞,邓晓军,彭涛,伊雄海,郭德华,韩丽,盛永刚,吴巧彬..亲水性相互作用色谱-四极杆串联线性离子阱质谱测定蛋白食品中L-羟脯氨酸残留[J].分析化学,2011,39(9):1373-1379,7.基金项目
本文系上海市科委技术标准专项(No.09DZ0503700)、长三角科技合作项目(No.10595812100)和质检公益性行业专项(No.201010073-04)和国家质检总局课题(No.2009IK314,2011 IK232)资助 （No.09DZ0503700）
