分析化学2011，Vol.39Issue(9)：1307-1312,6.DOI:10.3724/SP.J.1096.2011.01307
微流控芯片上大肠杆菌的电化学阻抗检测方法研究
Determination of E.coli with Electrochemical Impedance on Homemade Microfluidic Chip
摘要
Abstract
Based on the impedance properties of bacterial suspension and microfluidic impedance analytical technology on microchip, the homemade microfluidic impedance analytical system and a rapid and simple quantitative analytical method of bacterial were proposed. In the experiment, E. coli was taken as the detecting sample. Some parameters, such as amplitude of perturbation, conductivity of buffer, scanning frequency range and time of deposition, were optimized. The impedance signals of the standards and the composite samples of E. coli were measured by the homemade microsystem. Under the conditions of perturbation at 500mV, conductivity of buffer at 2. 40 μS/cm, scanning frequency ranged from 1 MHz to 100 Hz and so on, it was found that the quantitative relationship between the impedance and the concentration of the E. coli was in the range of 7. 43× 105 to 1. 49× 108 cfu/mL, and the detection limit was 7. 43×104 cfu/mL. In this study, more than 10 cfu E. coli in the testing region of the microchannel on the microchip could be detected. When the concentration of the E. coli was at 1. 39×107 cfu/mL, the RSD of the detection was 4. 75%(n = 5). This methodology was applied to detect E. coli in sewage, the results were basically the same as that of national standard method.关键词
大肠杆菌/微流控芯片/微流控电化学阻抗分析系统/定量检测Key words
E. coli/ Microfluidic chip/ Homemade microfluidic impedance analytical system/ Quantitative detection引用本文复制引用
彭金兰,徐溢,吴永杰,传娜,甘俊,田鹏..微流控芯片上大肠杆菌的电化学阻抗检测方法研究[J].分析化学,2011,39(9):1307-1312,6.基金项目
本文系全国博士学位论文作者专项资金基金(No.200941)、国家科技部国家科技部项目(No.2010ZX09401-306-1-5)、中央高校基本科研业务费(No.CDJXS 1-22-00-05)和重庆大学研究生科技创新基金项目(No.CDJXS 10-22-11-39)资助 （No.200941）
