分析化学2011，Vol.39Issue(9)：1313-1317,5.DOI:10.3724/SP.J.1096.2011.01313
基于二氧化硅球腔微电极阵列的过氧化氢生物传感器制备
Fabrication of Hydrogen Peroxide Biosensors Based on Microelectrode Array of Silicon Dioxide Cavities
摘要
Abstract
A silicon dioxide (SiO2) cavities array was fabricated on indium-tin oxide (ITO) electrode surface with the template of the polystyrene (PS) particles array by using sol-gel technique. The morphology of SiO2 cavities array was highly ordered which was obtained by scanning electrode microscope. The results of electrochemistrical study showed that the cyclic voltammetric (CV) curve of SiO2 cavities array was in accordance with that of microelectrodes array. Using hemoglobin (Hb) as model protein, an amperometric biosensor for detection of H2O2 was prepared by adsorbing Hb in SiO2 cavities directly. The properties of direct electrochemistry and electrocatalysis of Hb were studied by CV method. The response of the biosensor for H2O2 was fast. A linear relationship between current response and the concentration of H2O2 ranging from 2. 03 × 10~6 to 1. 21 × 10-2 mol/L was obtained with a detection limit of 5. 73×10-7mol/L. And the apparent Michaelis-Menten constant was 0. 266 mmol/L.关键词
溶胶-凝胶法/二氧化硅球腔/微电极阵列/血红蛋白/生物传感器Key words
Sol-gel technique/ Silicon dioxide cavities/ Microelectrode array/ Hemoglobin/ Biosensor引用本文复制引用
周丽娟,尹凡,周宇..基于二氧化硅球腔微电极阵列的过氧化氢生物传感器制备[J].分析化学,2011,39(9):1313-1317,5.基金项目
本文系国家自然科学基金(No.20975073),江苏省自然科学基金(No.BK2008147),常熟市2009年拔尖人才资助项目 （No.20975073）
