分析化学2011，Vol.39Issue(9)：1318-1322,5.DOI:10.3724/SP.J.1096.2011.01318
基于自动磁珠转运的转基因蛋白Cry1Ab检测
Magnetic Beads Transfer Based Assay for Cry1 Ab Protein
摘要
Abstract
A novel method for the immunoassay of genetically modified organisms (GMOs) protein CrylAb was proposed. First the nano-magnetic beads with the diameters around 400 nm were prepared by hydrothermal method. Then, the prepared magnetic beads were modified with -NH2 group using sol-gel approach. The antibodies were conjugated on the surface of magnetic beads through bi-functional agent glutaraldehyde. The immunoassay was carried out in the nucleic acid extractor which is commercial available. In this proposed method, the detection limit for CrylAb is less than 1 μg/L, which is comparable to the common ELISA methods. This automatic way is promising for its rapidity and simplicity.关键词
转基因作物/磁分离/Cry1Ab蛋白/磁珠转运/免疫检测Key words
Genetically modified organisms/ Magnetic separation/ CrylAb protein/ Magnetic beads transfer/ Immunoassay引用本文复制引用
李冬阳,樊凯,吴坚,应义斌..基于自动磁珠转运的转基因蛋白Cry1Ab检测[J].分析化学,2011,39(9):1318-1322,5.基金项目
本文系国家科技专项(No.2009ZX08012-004B)资助 （No.2009ZX08012-004B）
