分析化学2011，Vol.39Issue(9)：1341-1346,6.DOI:10.3724/SP.J.1096.2011.01341
在线免疫亲和净化-液相色谱-串联质谱快速测定中草药及中成药中10种真菌毒素
Determination of 10 Mycotoxins in Chinese Herbs and Chinese Medicines by Liquid Chromatography-Tandem Mass Spectrometry Using On-line Immunoaffinity Purifying
摘要
Abstract
Abstract A reusable on-line immunoaffinity purification column was prepared through packing multi-toxin immunoaffinity column filler in stainless column. The loading solution of on-line immunoaffinity purification column was phosphate buffered saline (PBS, pH 7. 4), the washing solution was water, and the transfering solution was (1∶ 1, V/V) acetonitrile-water. The mycotoxins in Chinese herbs and Chinese medicines were extracted by 75% methanol-PBS. The extract was directly injected into the on-line immunoaffinity column which was coupled with reversedphase analysis column. After high selective purification of the on-line immunoaffinity column, the mycotoxins were eluted onto analysis column for final separation and analysis. Ten mycotoxins: Toxin T2(T-2), Toxin HT 2(HT-2), fumonisin B1, fumonisin B2, ochratoxin A, zearalenone, α-zearalanol, β-zearalanol, α-zearalenol and β-zearalenol, could be detected by the method. The detection limits of the 10 mycotoxins were 1 -5 μg/kg. The average recoveries of three levels spiking were 62.3% -107.1%, and the relative standard deviation were ranged from 2.1% to 15.0%.关键词
在线免疫亲和净化/中草药/中成药/液相色谱-串联质谱/真菌毒素Key words
On-line immunoaffinity purification clean-up/ Chinese herbs/ Chinese medicine/ Liquid chromatography/tandem mass spectrometry/ Mycotoxins引用本文复制引用
赵孔祥,葛宝坤,陈旭艳,许泓..在线免疫亲和净化-液相色谱-串联质谱快速测定中草药及中成药中10种真菌毒素[J].分析化学,2011,39(9):1341-1346,6.基金项目
本文系国家质量监督检验检疫总局科研项目(No.20081K144)资助 （No.20081K144）
