分析化学2011，Vol.39Issue(9)：1459-1460,2.DOI:10.3724/SP.J.1096.2011.01459
高效液相色谱法分析马铃薯中α-茄碱
Detection of α-Solanine in Potato by High Performance Liquid Chromatography
摘要
Abstract
A method was established for the determination of α-solanine in potato by high performance liquid chromatog-raphy. The o-solanine was extracted from potato by refluxing extraction. The effects of different mobile phase composition, concentration, concentration ratio, flow speed, column temperature and other factors on a-solanine separation were studied. When the mobile phase was a mixture of acetonitrile and 0. 4% phosphoric acid aqueous solution, flow speed was 0. 8 mL/min, temperature was 30 ℃, detection wavelength was set at 210 nm, a-solanine could be separated well. The calibration of a-solanine was in good linearity in the range of 0. 16 - 0. 80 g/L. The average recovery was 96. 3% with relative standard deviation of 1.3% (n = 6).关键词
Potato/ a-Solanine/ High performance liquid chromatographyKey words
Potato/ a-Solanine/ High performance liquid chromatography引用本文复制引用
肖文军,李勤,熊兴耀,邹开静,张盛,陈凌,任国谱..高效液相色谱法分析马铃薯中α-茄碱[J].分析化学,2011,39(9):1459-1460,2.基金项目
本文系国家“十二五”支撑计划(No.2011BAD10B00)资助项且 （No.2011BAD10B00）
