分析化学2011，Vol.39Issue(9)：1451-1458,8.DOI:10.3724/SP.J.1096.2011.01451
脱氧核糖核酸甲基化分析方法在肿瘤诊断和治疗中的应用
Assay Methods of DNA Methylation and Their Applications in Cancer Diagnosis and Therapy
摘要
Abstract
DNA methylation catalyzed by DNA methyltransferase is an important epigenetic event that plays crucial roles in gene transcription and expression, genomic imprinting, embryogenesis, and maintaining normal cell function. Aberrant DNA methylation can lead to the development of cancer. Therefore, an approach for the rapid, facile, and sensitive detection DNA methylation could provide a powerful tool for early cancer disgnosis. This paper reviews the assay methods of DNA methylation. Specifically, direct sequencing, rnethylation-specific PCR, high performance liquid chromatographic method, capillary electrophoresis, fluorescence approach, electrochemical apparoach, and colorimetric approach are presented. In addition, the application of the assay of DNA methylation in cancer diagnosis and therapy is summarized. Finally, the further development on the assay methods of DNA methylation and their applications are briefly introduced.关键词
脱氧核糖核酸/甲基化/肿瘤诊断/肿瘤治疗/评述Key words
Deoxyribonucleic acid/ Deoxyribonucleic acid methylation/ Cancer diagnosis/ Cancer therapy/ Review引用本文复制引用
刘姝娜,屠蕴秋,李文,吴萍,张卉,蔡称心..脱氧核糖核酸甲基化分析方法在肿瘤诊断和治疗中的应用[J].分析化学,2011,39(9):1451-1458,8.基金项目
本文系国家自然科学基金(No.20833006),江苏省高校自然科学研究项目(Nos.09KJA150001,10KJB150009),江苏省凹土资源利用重点实验室开放课题(No.HPK201005),江苏省普通高校研究生科研创新计划(No.CX09B 307Z)和江苏省高校优势学科建设工程资助项目 （No.20833006）
