分析化学2011，Vol.39Issue(9)：1358-1362,5.DOI:10.3724/SP.J.1096.2011.01358
二醋酸纤维素-离子液体膜固定肌红蛋白制备过氧化氢传感器
Hydrogen Peroxide Biosensor Based on Cellulose Diacetate Ionic Liquid Film Immobilizing Myoglobin
摘要
Abstract
The myoglobin (Mb) was immobilized on the surface of glassy carbon electrode (GCE) by using composite of cellulose diacetate (CDA) and ionic liquid 1-butyl-3-methylimidazolium tetraflu-oroborate ([bmim]BF4). Spectroscopic analysis of the CDA-[bmim]BF4-Mb film showed that the Mb could retain its natural structure and bioactivity. The conductivity of the modified electrode had been greatly improved due to the doping of ionic liquid, which facilitated the electronic transfer of the CDA-[bmim]BF4-Mb membrane. Further experiment demonstrated that the Mb modified electrode exhibited an electrocatalytic activity for the reduction of H2O2. The catalytic peak current was linear to H2O2 concentration in the range of 5. 0-100 μmol/L with a detection limit of 2. 0 μmol/L. The apparent Michaelis-Menten constant (Km) was estimated to be 1. 758×10"-4 mol/L.ic关键词
肌红蛋白/二醋酸纤维素/离子液体/过氧化氢/电催化Key words
Myoglobin/ Cellulose diacetate/ Ionic liquid/ Hydrogen peroxide/ Electrocatalysis引用本文复制引用
董社英,谷广哲,于竹青,周元臻,汤宏胜,郑建斌..二醋酸纤维素-离子液体膜固定肌红蛋白制备过氧化氢传感器[J].分析化学,2011,39(9):1358-1362,5.基金项目
本文系西安建筑科技大学重大科技成果创新基金(No.ZC1004)、陕西省教育厅专项科研计划项目(No.11JK0570)及陕西省自然科学基础研究基金青年人才项目(No.2009JQ2011)资助 （No.ZC1004）
