工业微生物2011，Vol.41Issue(6)：11-14,4.DOI:10.3969/j.issn.1001-6678.2011.06.003
L-天冬氨酸-β-脱羧酶发酵条件的响应面优化
Optimization of fermentation conditions for L-aspartic acid -β-decarboxylase by response surface methodology
薛正莲 1高理所 1赵世光 1赵梦梦 1苏燕南1
作者信息
- 1. 安徽工程大学生物与化学工程学院,微生物发酵安徽省工程技术研究中心,安徽,芜湖,241000
- 折叠
摘要
Abstract
The Plackett-Burm an design and response surface analysism ethods w ere used to optin ize the ferm entation con ditions of L-aspartic acid -β decarboxy lase producing by Pseudom onas dacunhae XG-2-81. The com steep liquor, prion pep tides and m edium volum e w ere found to be the in portent factors. B ased on the experin en tel results of analysis of vari ance , the optin urn conditions w ere as follow s: 0 .64% com steep liquor, 1.21% prion peptides, 14 .71 % liquid vor um e. T he activity of L-aspartic acid-β-decarboxylase could reach 1107 μg / m L ? H by using the optin al fenn entation con ditions, w hich was increased by 10 .83% com pared w ith that by the original conditions.关键词
响应面设计法/L-天冬氨酸-β-脱羧酶/德阿昆哈假单孢菌/优化Key words
response surface m ethodobgy/ L-aspartic acid β decarboxylase/ Pseudom onas dacunha/ optim ization引用本文复制引用
薛正莲,高理所,赵世光,赵梦梦,苏燕南..L-天冬氨酸-β-脱羧酶发酵条件的响应面优化[J].工业微生物,2011,41(6):11-14,4.
