安徽农业科学2011，Vol.39Issue(21)：12641-12642,2.
水Z稻OsOle1基因的克隆及其遗传转化
Cloning and Genetic Transformation of OsOle1 Gene in Rice
姚清国 1李晓芹 1李晓兵 1段书德1
作者信息
- 1. 石家庄学院化工学院,河北石家庄050035
- 折叠
摘要
Abstract
[Objective] The cloning and transformation of rice OsOUl gene were conducted in the research. [Method] OsOUl gene waa cloned by RT-PCK. The amplified OsOlel was then ligased to pCAMBIA 1300 to construct CUS overexpression vector. Then the Agrobacuritm mediated method was used in rice callus transformation. [Result] The full-length of OsOUl gene was 498 bp and it encoded 189 amino acids. The over-expression vector Ub:: OtOel -CUS was prepared and transgenic pJants were successfully obtained. [ Conclusion ] The Iransgenic lines laid the foundation for the function research of OsOUl.关键词
水稻/基因克隆/遗传转化Key words
Rice/Gene cloning/Genetic transformation分类
生物科学引用本文复制引用
姚清国,李晓芹,李晓兵,段书德..水Z稻OsOle1基因的克隆及其遗传转化[J].安徽农业科学,2011,39(21):12641-12642,2.
