大豆科学2011，Vol.30Issue(4)：703-705,709,4.
野生大豆苯丙氨酸解氨酶(PAL)在不同诱导条件下变化规律
Response of Phenylalanine Ammonia-lyase (PAL)to Different Inducing Conditions in Wild Soybean (Glycine.soja)
摘要
Abstract
Wild Soybean(Glycine soja) with more desired traits is the ancestor of the cultivated soybean( Glycine max) ,and phenylalanine ammonia-lyase( PAL)is the key enzyme in the synthesis of isoflavones. With wild soybean (longye 01-177) as material,according to the influencing factors of PAL activities,selecting each 48 h for a cycle and sampling for each 3 h,we studied the enzyme activity, protein content and the variation of enzyme activity under the conditions of low temperature(-4°C:) (LT) .mechanical damage( MD) .ultraviolet ray( UV) ,hormone( BAP) and normal controls conditions. The results indicated that there was the same change trend of the enzyme activity and protein content of wild soybean PAL under different induction conditions and low temperature had the best induced effect.关键词
野生大豆/苯丙氨酸解氨酶/诱导条件Key words
Wild soybean(Glycine soja) /Phenylalanine ammonia-lyase( PAL) /Inducing conditions分类
农业科技引用本文复制引用
张必弦,胡小梅,朱延明,来永才,李炜,肖佳磊,李琬,毕影东..野生大豆苯丙氨酸解氨酶(PAL)在不同诱导条件下变化规律[J].大豆科学,2011,30(4):703-705,709,4.基金项目
国家科技支撑计划资助项目(2006AB13B05) （2006AB13B05）
农业部转基因新品种培育科技重大专项资助项目(2008ZX08009-003) （2008ZX08009-003）
黑龙江省青年科学基金资助项目(QC2010053). （QC2010053）
