安徽农业科学2011，Vol.39Issue(21)：12707-12709,3.
RT-PCR克隆广谱抗病基因NPR1及其蛋白表达载体构建
Cloning of Broad-spectrum Anti-disease Gene NPR1 with RT-PCR and Construction of Its Protein Expression Vector
摘要
Abstract
[Objective] It is to clone broad-spectrum anti-disease gene NPRl and to construct its protein expression vector. [Method] First extract Arabidopsis thaliana total RNA and design relevant primers, and then the method of reverse transcription PCR is adopted. With the method of enzyme ligation, this gene will be directed into protein expression vector. [ Result] After relevant testing, NPRl will be inserted into vector pMXBlO to obtain pMXBlO-NPRl protein expression vector. [Conclusion] Protein expression vector with NPRl will be successfully constructed.关键词
NPR1/广谱抗病/栽体构建Key words
Nonexpressor of pathogenesis-related genes 1/ Broad-spectrum anti-disease/ Construction of vectors分类
农业科技引用本文复制引用
刘永光,刘克锋,孙向阳..RT-PCR克隆广谱抗病基因NPR1及其蛋白表达载体构建[J].安徽农业科学,2011,39(21):12707-12709,3.基金项目
北京市教委面上项目(KM200910020014) （KM200910020014）
北京市园林绿化局治沙办项目(2008). （2008）
