安徽农业科学2011，Vol.39Issue(21)：12655-12657,3.
利用SYBR Green实时定量PCR法检测转基因植物外源基因的拷贝数
Estimating the Copy Number of Transgenes in Transformed Arabidopsis by SYBR Green Real-time Quantitative PCR
摘要
Abstract
[Objective] To explore the feasibility of using SYBR Green real-time quantitative PCR technique to estimate the transgene copy number of a transgenic plant. [ Method] Using SYBR Green real-time quantitative PCR technique, we have determined the copy numbers of the exogenous CYCD3 ; 1 in transgenic plants by comparing an endogenous single copy reference gene with CYCD3; 1 copy number in transgenic plants, meanwhile comparing CYCD3;1 copy numbers between wild plant and transgenic plants. [Result]The exogenous CYCD3;1 copy num bers calculated by this method is identical with results of traditional Southern blot analysis which is highly accurate. [ Conclusion ] This method is simple, effective and safe for estimating transgene copy numbers.关键词
转基因拟南芥/SYBR Green实时定量PCR/基因拷贝数Key words
Transgenic Arabidopsis/ SYBR green real-time quantitative PCR/ Gene copy number分类
农业科技引用本文复制引用
裘劼人,许颖,喻富根..利用SYBR Green实时定量PCR法检测转基因植物外源基因的拷贝数[J].安徽农业科学,2011,39(21):12655-12657,3.基金项目
国家自然科学基金项目(30270086). （30270086）
