大豆科学2011，Vol.30Issue(4)：537-540,4.
拟南芥胱硫醚-γ-合成酶( D-AtCGS)抗血清制备及对D-AtCGS转基因大豆的检测
Preparation of Polyclonal Antibody against D-AtCGS and Detection of D-AtCGS in Transgenic Soybean Plants
摘要
Abstract
Methionine( Met) has very important physiological functions as an essential sulfur-containing-amino acid. Cystathi-onine-γ-synthase (CGS) is the key enzyme in Met synthesis. In this study, prokaryotic expression vector of Arabidopsis D-AtCGS gene was constructed, and D-AtCGS protein was purified to prepare polyclonal antibody against D-AtCGS. Then transgenic soybean constitutive expressing or seeds specific expressing D-AlCGS and Lotus comiculatus transgenic plants with F-AtCGS or D-AtCGS obtained by Beijing Branch Center of National Center for Soybean Improvement were detected by Western blot method using polyclonal antibody we prepared. Results showed that detection signal of transgenic plants were all much stronger than wild type. At last, Western blot method for detection of transgenic soybean plants with AtCGS gene was successfully established. This work provides technology methods for analysis of CGS expression and detection of CGS transgenic plants.关键词
D-AtCGS:原核表达/抗血清制备/转基因大豆检测Key words
D-AtCGS/ Prokaryotic expression/ Preparation of polyclonal antibody/ Detection of transgenic plants分类
农业科技引用本文复制引用
王义鹏,于洋,孙石,许艳丽,侯文胜..拟南芥胱硫醚-γ-合成酶( D-AtCGS)抗血清制备及对D-AtCGS转基因大豆的检测[J].大豆科学,2011,30(4):537-540,4.基金项目
国家重大科技专项资助项目(2008ZX08010-004,2008ZX08004-003). （2008ZX08010-004,2008ZX08004-003）
