大豆科学2011，Vol.30Issue(4)：546-551,6.
大豆子叶节高频愈伤组织诱导及其植株再生
High Frequency Induction of Callus from Soybean Cotyledonary Node Explants and Its Plant Regeneration
摘要
Abstract
Effects of germination medium and different concentration ratios of BA and TDZ on the callus induction and plant regeneration were studied using fragments and slices of 7 day-germinated soybean cotyledonary nodes as explants. The result showed that the callus formation occurred from the cotyledonary node fragments only when it was cultured on germination medium containing BA. There were significant differences in callus induction frequencies among different explants, different concentration ratios of BA and TDZ on callus induction medium. The induction frequency of callus was low for cotyledonary node fragment explants (average less than 20% ) under the combination concentration of BA( 0. 05-0. 15 mg · L-1) and TDZ(0. 04-0.12 mg ? L-1) ,the highest induction frequency (50% ) was obtained when cotyledonary node fragments were cultured on callus induction medium containing 0. 15 mg ? L-1 BA and 0. 08 mg ? L-1 TDZ. However,the induction frequency of callus was significantly enhanced by using the cotyledonary node slices as explants (average more than 85% ) ,the highest frequency reached 95%. The callus induced was transferred to differentiation medium containing BA for shoot induction. The highest induction frequency of shoots (21.4% ) was obtained on differentiation medium containing 0.2 mg ? L-1 BA.关键词
大豆/子叶节切断/子叶节切片/愈伤组织/植株再生Key words
Soybean/Cotyledonary node fragment/Cotyledonary node slice/Callus/Plant regeneration分类
农业科技引用本文复制引用
孙明杰,魏玄,邹丹丹,赵春梅,蔡春梅,薛仁镐..大豆子叶节高频愈伤组织诱导及其植株再生[J].大豆科学,2011,30(4):546-551,6.基金项目
国家转基因生物新品种培育科技重大专项资助项目(2008ZX08010-002,2009ZX08010-009 B). （2008ZX08010-002,2009ZX08010-009 B）
