大豆科学2011，Vol.30Issue(4)：563-565,3.
转TaDREB3基因大豆基因漂流距离及频率的研究
Distance and Frequency of Gene Flow in Transgenic Soybean Overexpressing TaDREB3
摘要
Abstract
In order to investigate the ecological safety issues associated with transgenic soybeans planting,seeds of T4 generation of transgenic lines overexpressing TaDREB3, a stress-tolerant gene, were sowed in the field,surrounded with the nontransgenic control plant. The distance and frequency of gene flow in transgenic soybean overexpressing TaDREBi were determined. Two successive years' field and potted tests were carried out through glufosinate selection as well as molecular detection. The results showed that the optimal concentration of glufosinate selection in the trifoliate stage was 75 mg ? L-1. One positive plant was detected in the nontransgenic soybean at 1 m distance from the transgenic plant center,while no positive was detected in 3,5 and 8 m plants. Therefore, the possibility of TaDREBi gene flow under nature condition was 0.03478% with 1 m distance.关键词
转基因大豆/基因漂流/漂流距离/漂流频率Key words
Transgenic soybean/ Gene flow/ Distance of gene flow/ Frequency of gene flow分类
农业科技引用本文复制引用
张彬彬,李永光,盖江南,李文滨..转TaDREB3基因大豆基因漂流距离及频率的研究[J].大豆科学,2011,30(4):563-565,3.基金项目
转基因生物新品种培育重大专项资助项目(2008ZX08004-002). （2008ZX08004-002）
