大豆科学2011，Vol.30Issue(4)：566-568,3.
不同基因型大豆愈伤组织对农杆菌EHA105的敏感性研究
Sensitivity of Different Genotype Soybean Callus to Agrobacterium EHA105
摘要
Abstract
Soybean genotype has great impact on efficiency of genetic transformation. It is easy and convenient to evaluate the transgenic soybean by transient expression of GUS gene. This paper studied the sensitivity of eight genotypes to Agrobacterium EHA10S by GUS gene transient expression and staining times of GUS gene in soybean callus. The results showed that the sensitivity to Agrobacterium EHA10S varied with genotypes,the staining of Jilin47 and Dongnong42 were the best among the 8 soybean types indicated that soybean callus of Jilin47 and Dongnong42 were more sensitive to EHA105 ;the staining time was also varied with genotypes,appropriate GUS staining time was from 9 to 10 h.关键词
大豆/愈伤组织/基因型/GUS染色/农杆菌Key words
Soybean/Callus/Genotype/GUS staining/Agrobacteriem tumefaciens分类
农业科技引用本文复制引用
张庆林,赵艳,张艳,翟莹,苏连泰,王英,李景文,王庆钰..不同基因型大豆愈伤组织对农杆菌EHA105的敏感性研究[J].大豆科学,2011,30(4):566-568,3.基金项目
转基因生物新品种培育重大专项子课题(2008ZX08004-003) （2008ZX08004-003）
国家自然科学基金面上资助项目(30971 808) （30971 808）
吉林省科技发展计划重点资助项目(20080204) （20080204）
长春市科技局国际科技合作资助项目(08GH10) （08GH10）
“211”三期建设资助项目. （）
