安徽农业科学2011，Vol.39Issue(21)：12714-12718,12723,6.
大豆灰斑病菌菌丝块离体叶接种快速鉴定方法的建立
Establishment of a Rapid Identification Method to Determine Differences in Susceptibility of Cercospora Sojina Hara by Hyphae Inoculated on the Excised Leaf Surface
摘要
Abstract
[Objective] The aim was to establish a rapid identification method to determine differences in susceptibility of Cercospora Sojina Hara by hyphae inoculated on the excised leaf surface. [ Method ] 6 varieties and 2 races (race 1 and race 2) were used to study the establish ment of a rapid identification method to determine differences in susceptibility of Cercospora Sojina Hara by hyphae inoculated on the excised leaf. [ Result] The difference of lesion areas caused by the hyphae on Minimal medium was obvious under circumstances of 28 °C and 12 h light per day, and the results were in sympathy with reports. [ Conclusion ] The hyphae inoculation method owns the features of non-growing season restriction, non-circumstance restriction, simplicity and repeatability, so it could be an effective method in identifying the resistance to Cercospora Sojina Hara.关键词
大豆灰斑病/菌丝块/离体叶/接种/抗性Key words
Cercospora Sojina Hara/ Hyphae/ Excised leaf surface/ Inoculation/ Susceptibility分类
农业科技引用本文复制引用
张彪,黄军艳,刘胜毅,赵美霞,汪承刚,董彩华..大豆灰斑病菌菌丝块离体叶接种快速鉴定方法的建立[J].安徽农业科学,2011,39(21):12714-12718,12723,6.基金项目
大豆转基因专项(2009ZX08004-002B) （2009ZX08004-002B）
中国农业科学院油料研究所所长基金项目. （）
