安徽农业科学2011，Vol.39Issue(21)：12933-12935,3.
广西柳州产马尾松松针中莽草酸含量测定
Determination of the Content of Shikimic Acid in Leaf of pine needle in Liuzhou City of Guangxi Province
摘要
Abstract
[Objective]The determination of shikmic acid in pine needle and organic solvent extraction and separation method were established. [ Method ] The shikimic acid in leaf of pine needle was extracted and separated and its content was determined with the method of high performance liquid chromatography(HPLC) with the NH2 being taken as separation column;acetonitrile-2%H3P04(90:10) .mobile phase;the temperature of column,25 ℃ ;the flowing velocity,1 ml/min;and the testing wavelength,213 nm. [ ResultsJThe sample of shikimic acid extracted was with less impurity. The linear range of the shikimic acid was in 0.29 -2.88 μg(R2 =0.999 9) ,the average collection was 98.30% ,RSD was 0. 74% (n = 5). [ Conclusion ] The extraction and separation method was suitable for the determination of shikimic acid with high performance and environmental protection and low cost. The average content of shikimic acid in leaf of pine needle was 1.14%.关键词
莽草酸/新鲜马尾松松针/高效液相色谱Key words
Shikimic acid/ Fresh leaf of pine needle/ High performance liquid chromatography分类
医药卫生引用本文复制引用
粟本超..广西柳州产马尾松松针中莽草酸含量测定[J].安徽农业科学,2011,39(21):12933-12935,3.基金项目
广西生态工程职业技术学院基金资助项目(201002A). （201002A）
