安徽农业科学2011，Vol.39Issue(25)：15206,15235,2.
利用PBR322质粒监控MSAP甲基化检测过程
Monitoring the Procedures of MSAP Methyl Detection by PBR322 Plasmid
摘要
Abstract
[Objective] The research aimed to monitor the procedures of MSAP methyl detection, dominate its each reaction steps. [ Method] The enzyme digestion, ligation, preamplification and selective amplification in MSAP analysis were all monitored using PBR322 plasmid as the positive control. [ Result] The bands of PBR322 plasmid were limited in electrophoresis map, so MSAP methyl detection procedures could all be monitored according to whether or not there were bands and their suits, which was of help to find the error steps. [Conclusion] The study can provide the reliable safeguard for MSAP technology.关键词
PBR322质粒/MSAP甲基化检测/监控Key words
PBR322 plasmid/MSAP methyl detection/Monitoring分类
农业科技引用本文复制引用
彭海,席婷,张静,魏传斌..利用PBR322质粒监控MSAP甲基化检测过程[J].安徽农业科学,2011,39(25):15206,15235,2.基金项目
湖北省自然科学基金项目(2009CDB259) （2009CDB259）
中青年人才项目(020104504) （020104504）
武汉市高校科研项目(2008K006和2009K067). （2008K006和2009K067）
