安徽农业科学2011，Vol.39Issue(25)：15210-15212,3.
浙江红山茶总DNA提取及ISSR-PCR引物筛选
DNA Extraction and ISSR Primer Screening of Camellia chekiangoleosa
摘要
Abstract
[Objective] The aim was to screen out simple methods of DNA extraction and effective ISSR primers suitable to all germplasm materials of Camellia chekiangoleosa. [ Method] The modified CTAB method was used in the extraction of the genomic DNA,50 ISSR primers from other Camellia plants were used in the ISSR-PCR amplification for 20 germplasm materials from 10 populations in Fujian, Zhejiang, Jiangxi and Anhui Province. [Result] Pure DNA could be obtained rapidly by using the improved CTAB method, and the20 selected effective primers had rich polymorphism, clear bands and good repeatability. 337 DNA bands were obtained, of which 281 bands were polymorphic, accounting for 83.4% of the total amplified bands. And 16.85 bands could be amplified with a primer, averagely. [ Conclusion] The selected 20 primers could be effectively applied to ISSR analysis of the germplasm resources of C. Chekiangoleosa in Zhejiang.关键词
浙江红山茶/ISSR标记/引物筛选Key words
Camellia chekiangoleosa/ISSR marker/Primer screening分类
农业科技引用本文复制引用
谢云,李纪元,范正琪,朱高浦,周兴文..浙江红山茶总DNA提取及ISSR-PCR引物筛选[J].安徽农业科学,2011,39(25):15210-15212,3.基金项目
浙江省科技厅项目(2010C32043). （2010C32043）
