安徽医科大学学报2011，Vol.46Issue(11)：1113-1116,4.
TRAF6截短体的构建及其对NF-κB信号通路的影响
Construction of TRAF6 truncated gene expression vectors and their influence in the NF-κB pathway
摘要
Abstract
To construct the tumor necrosis factor receptor associated factor 6 ( TRAF6 ) truncated gene eukaryotic expression vectors, and identify influence in the activation of NF-kB pathway. Methods Using polymer-ase chain reaction and gene recombination techniques, four fragments deletions were amplified and cloned into pFlag-CMV2 vector respectively. The activity of these deletions in the NF-kB pathway were explored by the method of luciferase activity. Results Successfully cloned TRAF6 truncated genes into relevant vector. They were named: Nl ,N2,N3,C1. Conclusion The TRAF6 can activate the NF-kB pathway by N3 domain.关键词
NF-κB/TRAF6/荧光素酶报告基因Key words
NF-kappa B/ TRAF6/ luciferase activity assay分类
医药卫生引用本文复制引用
王敏,王晓辉,唐刘君,李长燕,董晓明,周扬帆,杨晓扬,杨晓明,汪思应..TRAF6截短体的构建及其对NF-κB信号通路的影响[J].安徽医科大学学报,2011,46(11):1113-1116,4.基金项目
国家重大科学研究计划(973计划)(编号:2011CB910600) （973计划）
