安徽医科大学学报2011，Vol.46Issue(11)：1144-1147,4.
免疫调节肽对T淋巴细胞转化和巨噬细胞分泌的影响
Effect of immunomodulating peptide on T lymphocyte transformation and macrophage secretion in BALB/c mice
摘要
Abstract
To study the effect of immunomodulating peptide ( PGPIPN ) deriving from β-cacein on spleen T lymphocyte transformation ( LT ) and murine bone marrow macrophage secretion ( BMMs ) in BALB/c mice. Methods The transformation effect of T lymphocyte with different concentrations of PGPIPN was detected by MTT method, and cytokines ( TNF-α and IL-6 ) released from murine BMMs by ELISA method. Results In vitro, LTs were markedly improved by ConA at 5 μg/ml and PGPIPN at different concentrations( P <0. 01 ). However, different concentrations of PGPIPN didn' t enhance spleen LT alone. The results also showed that PGPIPN with li-popolysaccharide ( LPS ) could stimulate BMMs to secrete TNF-a and IL-6, but PGPIPN alone was incapable of stimulating BMMs to release cytokines. Conclusion PGPIPN can promote LTs both in vivo and in vitro, and stimulate BMMs to release cytokines with LPS together in vitro. It suggests that PGPIPN can increase the immune effect of T lymphocytes and macrophages.关键词
脾T淋巴细胞/骨髓巨噬细胞/细胞因子/免疫调节肽Key words
spleen T lymphocyte/ murine bone marrow macrophage/ cytokine/ immunomodulating peptide分类
医药卫生引用本文复制引用
王巍,何晓光,郑欣,顾芳,秦宜德..免疫调节肽对T淋巴细胞转化和巨噬细胞分泌的影响[J].安徽医科大学学报,2011,46(11):1144-1147,4.基金项目
国家自然科学基金(编号:30872992) （编号:30872992）
