安徽农业科学2011，Vol.39Issue(21)：13085-13087,3.
以离子液体为介质生物胺的液相色谱测定
Determination of the Biogenic Amines in the Media of Ionic Solution with the Method of Liquid Chromatography
玉澜 1卢翠文 1谢济运1
作者信息
- 1. 柳州师范高等专科学校,广西柳州545004
- 折叠
摘要
Abstract
[Objective] The testing method of the biogenic amines ( Bas) in food and beverage was developed. [ Method ] The content of Bas in the food and beverage was tested with the dansly chloride as derivatization agent and the room temperature ionic liquid (RILT) as the medium of extraction,enrichment and derivatization. [Result]The testing process included the work of pre-column and chromatographic separation and the ideal condition of derivative was as follows; the dansyl chloride solution,1.5 ml;the buffer(Ph 9.10) ,2.0 ml and the RILT,1.0 ml,which was placed in ultrasonic cleaner for 30 minutes' treatment of ultrasound. Tlie extracted RILT after ultrasonic treatment was injected into the HPLC and six types of Bas were separated within 30 minutes with UV detector( A =254. 16 ran). Three procedures:derivatization,extraction and enrichment were combined in the testing method,which was greatly simple. The ionic solution could effectively promote the separation with good stability and the method was also with good liner range. [Conclusion]This method was quick,simplicity and easy to be operated.关键词
室温离子液/生物胺/高效液相色谱Key words
Room temperature ionic liquid/Biogenic amines/HPLC分类
农业科技引用本文复制引用
玉澜,卢翠文,谢济运..以离子液体为介质生物胺的液相色谱测定[J].安徽农业科学,2011,39(21):13085-13087,3.
