安徽农业科学2011，Vol.39Issue(24)：14531-14533,11544,4.
流加培养方式对大肠杆菌XD-12发酵的影响研究
Study on the Effects of Fed-batch Culture Method of Escherichia coli XD-12 Fermentation
摘要
Abstract
[Objective] The research aimed to study the fed-batch culture method of Escherichia coli and enhance the fermentation concentration of transaminase donor.E. coli XD-12. [Method] The effects of carbon source feeding, nitrogen source feeding,feeding with pH control on E. coli fermentation were studied to obtain the optimal culture conditions. [ Result] The optimal cultural conditions lot transaminase-produeing E.coli were as Mows; temperature of 37 ℃ .agitation speed of 400 r/min,aeration quantity of 1.5 L/min,initial pH of medium of 7.0,for controlling fermentation process pH of 7.5,initial glucose concentration of 5 g/L,initial nitrogen source of 5 g/L peptone + 1.5 g/L beef extract, 120 g/L glucose solution was intermittently fed at an interval of 2 h after glucose concentration declined to 2 g/L And 15 g/L peptone and 4. 5 g/L beef extract were intermittently fed at an interval of 2 h after 8 hours. After culture 24 h under these conditions, the cell dry weight concentration of E.coli reached 9.66 g/L,104.7% higher than that in batch culture. [ Conclusion] This research was of an important realistic significance for reducing the production cost of preparing L-phenylalanine by enzyme method,enhancing the production efficiency of L-phenylala-nine,sufficing the increasing market requirements of L-phenylalanine.关键词
流加培养/大肠杆菌/发酵浓度Key words
Fed-batch culture/Escherichia coli/Fermentation concentration分类
农业科技引用本文复制引用
潘自皓,顾薇,潘扬..流加培养方式对大肠杆菌XD-12发酵的影响研究[J].安徽农业科学,2011,39(24):14531-14533,11544,4.基金项目
江苏省“青蓝工程”项目(2008年) （2008年）
江苏省高校优势学科建设工程项目(2011年). （2011年）
