广西科学2011，Vol.18Issue(3)：269-272,4.
一株耐高温L-乳酸高产菌的选育及其发酵特征
Breeding and Characterization of a Thermotolerant and High-yield Lactobacillus rhamnosus for L-Lactic acid Production
摘要
Abstract
By UV mutagenesis.a high-yield and thermostable L-lactic acid mutant strain-GX-6 was originally obtained from Lactobacillus rhamnosus JCM1553. At 47'C ,the maximum L-lactic acid yield of GX-6 in glucose was 117g/L for 56h,which was 17% higher than that of the o-riginal strain(100g/L) ,with a conversation rate of 68. 8 %. The maximum L-lactic acid yield was 138g/L for 72h under the same condition in cassava starch,77. 6% higher than that of the original strain(73g/L) and the conversation rate reached to 85. 4%. After subculture for 16 generations,the L-lactic acid production capacity of GX-6 remains unchanged, indicating that the mutant is stable.关键词
鼠李糖乳杆菌/紫外诱变/高温L-乳酸发酵Key words
Lactobacillus rhamnosus, UV mutagenesis,L-lactic acid fermentation at high temperature分类
化学化工引用本文复制引用
李检秀,孙靓,吴军华,郭铃,孙菲菲,黄艳燕..一株耐高温L-乳酸高产菌的选育及其发酵特征[J].广西科学,2011,18(3):269-272,4.基金项目
国家科技支撑计划项目(2007BAD75B06),广西科学基金项目(桂科攻0782003-4),广西科学院基本科研业务费项目(10YJ25SW11)资助. （2007BAD75B06）
