南方农业学报2011，Vol.42Issue(8)：874-877,4.
壳寡糖诱导甘蔗叶多酚与防御酶活性的变化
Chitosan oligosaccharide induced changes in polyphenols and defense enzymes activities in sugarcane leaves
摘要
Abstract
[ Objective ]The present study was carried out to explore the effect of chitosan oligosaccharide on secondary metabolites and some defense enzyme activities related to stress resistance in sugarcane. [ Method ]The leaves of sugarcane variety ROC22 were treated with 0 (CK), 25, 50 and 75 mg/L chitosan oligosaccharide solution at the early elongation stage. The polyphenol content and the activities of defense enzymes, viz., phenylalanine ammonialyase (PAL), polyphenol oxidase (PPO) and peroxidase (POD) were measured in +1 leaf of sugarcane at 1, 2, 3 and 4 d after spraying. [Result] 50-75 mg/L chitosan oligosaccharide treatment was conducive to the accumulation of polyphenol in sugarcane leaf. The PAL activity reached to its maximum and was 1.78 times higher than the control after 2 d of 50 mg/L chitosan oligosaccharide treatment. The PPO activity reached to its maximum and was 1.7 times higher than the control after 4 d of 50 mg/L chitosan oligosaccharide treatment. The 75 mg/L chitosan oligosaccharide treatment was conducive to improve POD activity of sugarcane leaf. [ Conclusion ]It was found that 50-75 mg/L chitosan oligosaccharide treatment can improve the polyphenol accumulation and the activities of defense enzymes in sugarcane leaves, which is helpful for improving sugarcane resistance to various biotic and abiotic stresses.关键词
甘蔗/壳寡糖/多酚/苯丙氨酸解氨酶/多酚氧化酶/过氧化物酶Key words
sugarcane / chitosan oligosaccharide/ polypheols/ phenylalanine ammonialyase (PAL)/ polyphenol oxidase (PPO)/ peroxidase (POD)分类
农业科技引用本文复制引用
周桂,靳晓芸,邓光辉,丘立杭,邹成林,李杨瑞..壳寡糖诱导甘蔗叶多酚与防御酶活性的变化[J].南方农业学报,2011,42(8):874-877,4.基金项目
国家科技支撑计划项目(2007BAD30B03) （2007BAD30B03）
广西科技攻关重点项目(桂科攻0782004-3) （桂科攻0782004-3）
广西科技攻关项目(桂科能0815011) （桂科能0815011）
广西自然科学基金项目(2010GXNSFA013101) （2010GXNSFA013101）
广西教育厅项目(200707MS034) （200707MS034）
广西民族大学引进人才项目(2010QD022) （2010QD022）
