安徽农业科学2011，Vol.39Issue(24)：14896-14898,3.
西藏黄牛胎儿成纤维细胞的分离与培养
Isolation and Culture of Fetal Fibroblasts of Tibetan Yellow Cattle
摘要
Abstract
[Objective ] To isolate fetal fibroblasts from Tibetan yellow cattle and thus to provide the ideal biological material for cloning and cel-iular biology of animals inhabiting in the Tibet Plateau. [Method] The fibroblast cells were isolated from Tibetan yellow fetus by explant culture method. The subculture, morphology and dynamics were observed. [ Result ] The fetal fibroblasts of Tibetan yellow cattle had typical morphology of fibroblast cells. The isolated cells had three growth stages including retention period (0-2 d) , logarithmic phase (2-7 d) and platform phase (7 -8 d). Its doubling time was 60 h. [Conclusion] Fetal fibroblasts of Tibetan yellow cattle have been successfully cultured.关键词
西藏黄牛:胎儿成纤维细胞/体外培养/分离Key words
Tibet yellow cattle/ Fetal fibroblasts/In vitro culture/ Isolation分类
农业科技引用本文复制引用
任子利,赵彦玲,王建洲,李瑜鑫,强巴央宗..西藏黄牛胎儿成纤维细胞的分离与培养[J].安徽农业科学,2011,39(24):14896-14898,3.基金项目
国家自然科学基金资助项目(31040077) （31040077）
211工程师资队伍建设项目资助(SZRC-211-01) （SZRC-211-01）
西藏科技厅地区自然科学基金资助项目(2010-17-02). （2010-17-02）
