南方农业学报2011，Vol.42Issue(8)：991-994,4.
猪繁殖与呼吸综合征病毒变异株和经典株RT-PCR鉴别诊断技术及其临床应用
RT-PCR differential diagnosis technique for variant and classical strains of PRRSV and its clinical application
摘要
Abstract
[Objective]The studies were conducted to establish the RT-PCR assay for the differential diagnosis of the highly pathogenic porcine reproductive and respiratory syndrome virus (PRRSV) (variant strain) and classical PRRSV in order to provide control measures for PRRS. [Method]A pairs of specific primers was designed on the basis of Nsp2 gene sequences of PRRSV collected from GenBank to amplify PRRSV genes using RT-PCR for differentiating the variant and classical strains of PRRSV. The specificity and sensitivity test were performed and clinical applications were carried out. [Result]The fragments of 332 and 241 bp were amplified from classical and variant strains of PRRSV using RT-PCR, respectively , which could be differentiated on the basis of their sizes. The sensitivity test results demonstrated that as little as 100 fg of the classical strain of PRRSV RNA, and 10 fg of the variant strain of PRRSV RNA could be detected by RT-PCR. The clinical application of RT-PCR showed that, out of 37 suspicious disease samples, 24.32 and 51.35% belonged to classical and variant strains of PRRSV, respectively, while the others showed negative. [Conclusion ]The established RT-PCR differential diagnosis technique was found to differentiate the classical and variant strains of PRRSV effectively, and showed highly specific and simple assay, which may be widely used in clinical diagnosis.关键词
猪繁殖与呼吸综合征病毒(PRRSV)/经典株/变异株/RT-PCRKey words
PRRSV/ classical strain/ variant strain/ RT-PCR分类
农业科技引用本文复制引用
梁民,蒋柳平,黄溢泓,盘美妮..猪繁殖与呼吸综合征病毒变异株和经典株RT-PCR鉴别诊断技术及其临床应用[J].南方农业学报,2011,42(8):991-994,4.基金项目
广西柳州市科学基金项目(2008060605) （2008060605）
