安徽农业科学2011，Vol.39Issue(25)：15230-15232,3.
小球藻鉴定基因的筛选及高产油微藻的分子鉴定
Screening of the Gene for Chlorella Identification and Identification of Oil-producing Microalgae
摘要
Abstract
[Objective] The aim was to select suitable gene for Chlorella identification and to identify the oil-producing microalgae. [Method] Four candidate gene sequences, the nuclear genomic rDNA of the 18S rRNA gene, internal transcribed spacer (ITS), internal transcribed spacer Ⅱ (ITS Ⅱ ) and the chloroplast rbcL gene, were selected for Chlorella molecular identification. Through these four candidate genes, the genetic variability and distinguish ability between intra-species and inter-species was analyzed to choose the right genes for identification of the high oil-content Chlorella. On this basis, application of these gene segments were classified and identified for five fresh-water isolated Chlorella, which oil-content is more than 30%. [Result] ITS gene was a suitable gene because of its high variation and short fragment length, meanwhile its genetic distance intra-species (0.439 6 ± 0.135 9) was larger than inter-species (0.045 7 ± 0.084 3). Its sequence length varied between different species whereas highly conserved in the same species. By the application of ITS sequences, respectively, five high oil-content stains were identified as one C. Vulgaris, two strains of C. Sorokiniana and two strains of algae Chlorella sp. [ Conclusion] This study had provided reference for the establishment of identification gene pool of Chlorella.关键词
产油微藻/分子鉴定/内转录间隔区/18S rRNA基因/遗传距离Key words
Oil-producing microalgae/Molecular identification/Internal transcribed spacers/18S rRNA gene/Genetic distance分类
农业科技引用本文复制引用
何茹,刘君寒,王士安,李福利..小球藻鉴定基因的筛选及高产油微藻的分子鉴定[J].安徽农业科学,2011,39(25):15230-15232,3.基金项目
中国科学院知识创新工程重要方向性项目(KGCX2-YW-374-3) （KGCX2-YW-374-3）
山东省科技攻关项目(2008GG20007002). （2008GG20007002）
