安徽农业科学2011，Vol.39Issue(25)：15272-15274,3.
大孔树脂对黄芪总皂苷的分离纯化研究
Study on the Separation and Purification of Total Saponins from RADIX ASTRAGALI by Macroporous Resin
摘要
Abstract
[Objective]To screen the optimum conditions for separation and purification of total saponins from RADIX ASTRAGALI. [Method] The content of total saponins in RADIX ASTRAGALI was determined by ultraviolet spectrophotometry using dynamic adsorption - desorption method aimed at studying the optimum conditions for separating and purifying total saponins from RADIX ASTRAGALI with macroporous resin. [Result] The optimum conditions for separation and purification of total saponins with AB-8 were as follows; extracting concentration of 0.1 g/ml, sampling volume of 3 BV, flow rate of 1.0 ml/min, washing with 4 BV of distilled water first and then desorption with 4 BV of 50% ethanol. Under the above condition, the yield of total saponins reached as high as 52. 1%. [Conclusion]This method was simple and feasible, which could meet industrial requirements.关键词
黄芪(RADIX ASTRAGALI)/总皂苷/大孔树脂/紫外分光光度法Key words
RADIX ASTRAGALI/Total saponins/Macroporous resin/Ultraviolet spectrophotometr分类
农业科技引用本文复制引用
李春红,田吉,何兵,肖顺汉..大孔树脂对黄芪总皂苷的分离纯化研究[J].安徽农业科学,2011,39(25):15272-15274,3.基金项目
四川省教育厅重点项目(2005 A76). （2005 A76）
