安徽农业科学2011，Vol.39Issue(25)：15384-15386,3.
检测H9亚型AIV的压电免疫传感器的研究
Study on the Piezoelectric Immunosensor for the Detection of H9 Subtype Avian Influenza Virus
摘要
Abstract
[Objective] The aim is to develop the piezoelectric immunosensor to detect H9-subtype avian influenza virus(AIV). [Method] The immunosensor was set up by self-assembling mercaptopmpionic acid( MPA) to be monolayer on the silver-coated electrode of quartz crystal and coupling the monoclonal antibody to H9 subtype AIV with N-ethy-N'-(3-dimethyl aminopropyl)carbodiimide hydrochloride(EDC) and N-hydroxysuccinimide(NHS). The immunosensor to detect H9 subtype AFV was established. [Result] The results showed that the immunosensor displayed better specificity to H9 AIV and had no response to H5AIV and NDV when it was used for detection. The sensitivity test indicated the detection sensitivity for the H9 subtype AIV could reach 20 - 100 EID50. [ Conclusion] The research provided a foundation for further research on the immunosensor for detecting AIV and it could be a new approach to detect other viruses in animal husbandry.关键词
压电免疫传感器/生物自组装法/H9亚型禽流感病毒Key words
Piezoelectric immunosensor/Biological self-assembly method/H9 subtype avian influenza virus分类
农业科技引用本文复制引用
刘靖清,卞红春,胡云发,王新卫,陈枝楠,詹爱军..检测H9亚型AIV的压电免疫传感器的研究[J].安徽农业科学,2011,39(25):15384-15386,3.基金项目
“十一五”国家科技支撑项目(2006BAK20A29)和广东省科技重大专项计划项目(2004A2090102). （2006BAK20A29）
