安徽农业科学2011,Vol.39Issue(25):15314-15316,3.
转双价基因(Bt+CpTI)抗虫棉对棉蚜羧酸酯酶和乙酰胆碱酯酶比活力的影响
Effect of Transgenic Bt plus CpTI Cotton on Carboxylesterase and Acetylcholinesterase of Cotton Aphid Aphis gossypii
摘要
Abstract
[Objective] The research aimed to assess the effect of transgenic Bt plus CpTI cotton variety SGK321 on carboxylesterase and ace-tylcholinesterase of cotton aphid Aphis gossypii and give theoretical basis for studying the biosafety of transgenic cotton. [ Method] Cotton a-phids were fed with both SGK321 and Shiyuan321 (normal parental varieties) for over 40 generations. Enzyme activities were compared between cotton aphids fed on SGK321 for 1, 2, 3, 41, 42 and 43 generations with those on Shiyuan321. [ Result] The carboxylesterase activities of cotton aphids fed on SGK321 for 1 generation was significantly higher than those fed on Shiyuan321. Acetylcholinesterase activities of cotton aphids fed on SGK321 for 1, 2 and 3 generations were significantly higher than those fed on Shiyuan321 in the same generation. But there was no significant difference of enzyme activities among cotton aphids fed on SCK321 for long run, and those on parental cotton. [Conclusion] The cotton aphid that fed on transgenic Bt plus CpTI cotton SGK321 for the long run have applicability about SCK321 through the accommodation of Detoxifying Enzyme .关键词
转双价基因棉/棉蚜/羧酸酯酶/乙酰胆碱酯酶Key words
Transgenic Bt plus CpTI cotton/Aphis gossypii Glover/Carboxylesterase/Acetylcholinesterase分类
农业科技引用本文复制引用
薛丽,声延,周正堂,高希武,宋敦伦..转双价基因(Bt+CpTI)抗虫棉对棉蚜羧酸酯酶和乙酰胆碱酯酶比活力的影响[J].安徽农业科学,2011,39(25):15314-15316,3.基金项目
转基因生物新品种培育重大专项(2008ZX08012-04). (2008ZX08012-04)