安徽农业科学2011，Vol.39Issue(25)：15370-15372,3.
猪H-FABP基因内含子1的一个遗传多态性及其遗传效应分析
A Novel Genetic Polymorphism and Its Genetic Effects of Porcine Heart Fatty Acid-Binding (H-FABP) Gene in Intron 1
摘要
Abstract
[Objective] The aim was to provide basic reference for the use of H-FABP gene in marker-assisted selection of the breeding process of pig. [Method] Single-nucleotide polymorphisms of the H-FABP gene in Shanxi White pig,Mashen pig,Large White pig.Landrace and Duroc were tested by PCR-SSCP, and the correlation between genotype and intramuscular fat content in pigs were analyzed. [ Result] One polymorphism was found in the amplified region of intron 1 of porcine H-FABP gene,in which two alleles (A and 5) and three genotypes( AA, AB, and SB) were examined. C→T transition was detected by sequencing the homozygotes. The multiple comparison of the distribution of genotype in different pig varieties revealed that Mashen pig showed extremely significant difference ( P＜0. 01) in genotype distribution with Shanxi White, Landrace, Large White and Duroc breeds; whereas no significant differences (P＞0.05) were found in genotype distribution between other breeds. Based on the fixed effect model,extremely significant differences (P＜0.01) were found in the intramuscular fat content among different H-FABP genotypes. Using least square analysis,it was found that there was significant differences (P ＜0.05) in the intramuscular fat content between the individuals of the BB genotypes and those of the AA genotypes. [ Conclusion] The H-FABP genotype had significant effects on the meat quality.关键词
H-FABP基因/猪/遗传多态性/遗传效应Key words
H-FABP gene/Pig/Genetic polymorphisms/Genetic effects分类
农业科技引用本文复制引用
杨文平,李彩桃,高爽,王明艳,张红梅,李超,曹果清,周忠孝..猪H-FABP基因内含子1的一个遗传多态性及其遗传效应分析[J].安徽农业科学,2011,39(25):15370-15372,3.基金项目
河北联合大学科研启动项目(07101168). （07101168）
