安徽农业科学2011，Vol.39Issue(25)：15779-15782,15803,5.
超声波法提取香蕉皮多糖的条件优化及其生物活性
Optimization of Extraction for Banana Peel Polysaccharides and Its Bioactivity
摘要
Abstract
[Objective]To optimize the best extraction conditions of polysaccharides from dried banana peel and to investigate its bioactivity. [Method]Base on the single factorexperiments,factors such as material to solvent ratio,extracting time,extraction temperature and extractiom times were optimized by ultrasonic assistant,and the phenol - sulfuric reaction was used for the determination of polysaccharide. MTT assay was used to study the effect of banana peel polysaccharides on breast cancer MCF_7 cells. [ Result ] The optimum process conditions for polysaccharides were:ultrasonic power 320 W,ultrasonic time 210 s,extraction temperature 40℃ and solidtosolvent ratio 1:50 andextracting fouir times. [ Conclusion ] Ultrasonic extraction of polysaccharides from banana peel is efficient and simple, the method is stableand reliable. The banana peel polysaccharide can inhibit the proliferation of breast cancer MCF_7 cells.关键词
香蕉皮/多糖/超声提取Key words
Banana peel/Polysaccharides/Ultrasonic extraction分类
农业科技引用本文复制引用
朱开梅,顾生玖,唐世锭,刘建楠,欧奕,赵磊..超声波法提取香蕉皮多糖的条件优化及其生物活性[J].安徽农业科学,2011,39(25):15779-15782,15803,5.基金项目
广西科技攻关项目(桂科攻0815005-1-17、0992003 A-25) （桂科攻0815005-1-17、0992003 A-25）
广西科技特派员专项(桂科攻09321056、09321052) （桂科攻09321056、09321052）
桂林市科技攻关项目(科技攻关20090106-6 （）
20080103-5). （）
