福建农业学报2011，Vol.26Issue(3)：365-370,6.
朱砂根成年茎段的离体培养研究
In vitro Culture of Adult Ardisia crenata Stems
摘要
Abstract
Adult stems of Ardisia crenata were used to establish sterile lines for studying the in vitro reproduction processes including the axillary bud induction, bud proliferation, subculture and rooting. Tumor-like seedlings occurred in nature, as well as in vitro, were used for the cytological observation. The results showed that (1) MS medium containing cytokinin and NAA could induce axillary buds from the adult stems; (2) KT medium containing GA and NAA benefited A. Crenata seedling proliferation at the optimal application of 3 mg · L-1KT; (3) addition of Vc or activated charcoal during the subculture process helped reduce browning, and that of A. Crenata leaf homogenate shortened growth time during the recovery; and (4) reduction in inorganic salt strengthened the seedling development and rooting, while appropriate increase on growth hormone and decrease on mitogen concentration led to even more desirable results. Our cytological observation indicated that the suberization or irregular multi-frequency division of external cells of the growing point resulted in the tumor-like seedling's failure to form organ primordia, significantly affecting the growth of natural seedlings and impeding the seedling's in vitro proliferation rate.关键词
朱砂根/离体培养/石蜡切片/瘤状苗Key words
Ardisia crenata / in vitro cultures paraffin section/ tumor-like seedlings分类
农业科技引用本文复制引用
邓素芳,杨旸,赖钟雄..朱砂根成年茎段的离体培养研究[J].福建农业学报,2011,26(3):365-370,6.基金项目
国家科技支撑计划项目(2007BAD07B03) （2007BAD07B03）
福建省武平县政府及林业局科技项目(2005) （2005）
