哈尔滨商业大学学报（自然科学版）2011，Vol.27Issue(4)：513-516,4.
吴茱萸碱调控微管蛋白聚集阻滞HepG -2细胞周期
Study on effect on tubulin induced by evodiamine and its mechanism in HepG-2 cell line
高世勇 1刘溪 2季宇彬1
作者信息
- 1. 哈尔滨商业大学生命科学与环境科学研究中心,哈尔滨150076
- 2. 国家教育部抗肿瘤天然药物工程研究中心,哈尔滨150076
- 折叠
摘要
Abstract
To study the effect of cells cycle arrest and mechanism in human liver cancer HepG-2 which induced by evodiamine. The cytotoxic effect of evodiamine was determined by MTT assay. The influence of evodiamine on human liver cancer' s cell cycle distribution was examined by flow cytometry. Used immunofluorescence and observed by confocal laser microscopy to investigate the changes of tubulin. Treated HepG-2 cell line with different doses of evodiamine ,for 24 hours, the IC50 was 19.62 μmol/L. Evodiamine could induce G2/M phase arrest. Under confocal laser microscopy, the polymeric form of tubulin was changed significantly, blocked the spindle poles formed. Evodiamine could induce cell cycle arrest in human liver cells line HepG-2, the mechanism had effect on polymeric form of tubulin and play the role of anticancer activity.关键词
吴茱萸碱/细胞周期/人肝癌细胞HepG-2/微管蛋白α-tubulinKey words
evodiamine/ cell cycle/ human liver cancer HepG-2 cells line/ α-tubulin分类
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高世勇,刘溪,季宇彬..吴茱萸碱调控微管蛋白聚集阻滞HepG -2细胞周期[J].哈尔滨商业大学学报（自然科学版）,2011,27(4):513-516,4.
