大连工业大学学报2011，Vol.30Issue(4)：235-237,3.
麦冬皂苷D的分离提纯
Separation and purification of ophiopogonin D
摘要
Abstract
18.1g ophiopogon total saponins were extracted from 10 kg Ophiopogon japonicus by methanol, the yield of which is 0. 18%. Ophiopogon total saponins were separated on silica gel column using chloroform-methanol as eluting agent at different gradient and 2. 31 g ophiopognin D was obtained, the yield of which is 12. 8%. HPLC analysis was used to detect the purity of ophiopogonin D, the purity was not less than 91. 2%. The result proved that silica gel column could quickly isolate high purity ophiopogonin D.关键词
麦冬皂苷D/薄层层析/硅胶柱层析/高效液相色谱Key words
ophiopogonin D/TLC/silica gel column/HPLC分类
农业科技引用本文复制引用
邓惠方,王东明,富瑶瑶,胡雪莲,鱼红闪,金凤燮..麦冬皂苷D的分离提纯[J].大连工业大学学报,2011,30(4):235-237,3.基金项目
国家自然科学基金资助项目(30470055) （30470055）
辽宁省教育厅创新团队项目(2007T006,2008T008,2009T007). （2007T006,2008T008,2009T007）
