农业生物技术学报2011，Vol.19Issue(4)：692-697,6.DOI:10.3969/j.issn.1674-7968.2011.04.014
SYBR Green I荧光定量PCR法检测朗德鹅填饲前后黑素皮质素受体-4基因(MC4R)的表达
The Melanocortin-4 Receptor Gene (MC4R) Expression Detected By SYBR Green I Real-time Quantitative PCR in Landes Geese before and after Overfeeding
摘要
Abstract
The expression characterization of Melanocortin-4 Receptor Gene (MC4R) before and after overfeeding plays an important role in fat metabolism of Lades geese (Casuarius caswrius). SYBR Green I Real-time quantitative PCR was developed to analyze the expression characterization of MC4R gene in 13 tissues. PCR results showed that both MC4R and β-actin were expressed in all 13 tissues before and after overfeeding of Landes goose. SYBR Green I Real-time quantitative PCR results showed that the relative expression of MC4R in kidney, intestine and heart after overfeeding was lower than those before overfeeding, their 2-Δδo values were 0.41 ±1.80, 0.65±5.75 and 0.72±1.22, respectively. But the relative expressions of MC4R in other tissues were all higher after overfeeding,their 2-Δδo values from high to low were 22.78±1.00 (brain), 9.08±2.80 (liver), 5.28±1.83 (lung), 3.78±3.01 (pancreas), 3.07±3.64(leg muscle), 2.90±0.97 (stomach), 2.34±1.66 (subcutaneous adipose), 2.18±3.01 (abdominal adipose), 2.07±0.37 (chest muscle) and 2.01±1.75 (pleen). We conclude that the results are consistented with the mechanism of MC4R and can provide indirect basis for researches on animal production and human obesity.关键词
黑素皮质素受体4基因(MC4R)/脂肪代谢/鹅/实时荧光定量PCR/表达Key words
Melanocortin-4 receptor gvae(MC4R)/Fat metabolism/Geese/Real-time quantitative PCR/Expression引用本文复制引用
原昊,张红艳,田勇,沈军达,王芳,弓彦,王德前,石放雄,卢立志..SYBR Green I荧光定量PCR法检测朗德鹅填饲前后黑素皮质素受体-4基因(MC4R)的表达[J].农业生物技术学报,2011,19(4):692-697,6.基金项目
本研究由国家重点国际合作项目(No.2008DFA31060)和浙江省重大科技攻关项目(No.2007C12058)共同资助 （No.2008DFA31060）
