新疆农业科学2011，Vol.48Issue(7)：1307-1312,6.
扩展莫尼茨绦虫蛋白激酶C相互作用蛋白(PICK1)基因的克隆及序列分析
Cloning and Sequence Analysis of the cDNA Encoding Protein Interacting with C Kinase 1 (PICK1) in Moniezia expansa
摘要
Abstract
[Objective] The purpose of this program was to clone and identify novel genes from an adult Monieda expansa (M. Expansa) cDNA library, and provide a foundation for further research. [Method]A cDNA library was constructed from M. Expansa adult stage. Clones were selected randomly from the cDNA library and were sequenced by using the method of expression sequence tags (ESTs) . Novel genes were acquired by primer -walking. The cDNA sequence encoding M. Expansa PICK1 protein was analyzed, including searching the ORF, translating the nucleotide to protein sequence, similarity searches and secondary structure predication with bioinformatics analysis. [ Result ] PICK 1 genes, 1527 bp and coding for 447 amino acids, was cloned and sequenced, then the sequence was submitted to GenBank and got an accession number, GH291479. The theoretical pi was 5.22 and molecular weight was SO. 173 3 ku. [Conclusion]The full - length cDNA encoding M. Expansa PICK1 was obtained, which laid the foundation for further functional study of this gene.关键词
扩展莫尼茨绦虫/PICK1/序列分析Key words
M. Expansa/, PICK1/sequence analysis分类
农业科技引用本文复制引用
赵文娟,康立超,薄新文,王新华..扩展莫尼茨绦虫蛋白激酶C相互作用蛋白(PICK1)基因的克隆及序列分析[J].新疆农业科学,2011,48(7):1307-1312,6.基金项目
国家重点基础研究发展计划“973”前期研究专项(2006C708512) （2006C708512）
新疆农垦科学院青年基金(YQJ201110) （YQJ201110）
