新疆农业科学2011，Vol.48Issue(7)：1317-1323,7.
结核分枝杆菌三价DNA疫苗在奶牛体内诱导的免疫应答
Immune Response Induced by the Trivalent DNA Vaccine from Mycobacterium tuberculosis in Cows
摘要
Abstract
The purpose of this study was to evaluate the immune response induced by DNA vaccine based on Mycobacterium tuberculosis Ag85B, MPT83 and MPT64 antigen in cow. Six months old Holstein Cows were immunized with the eukaryotic expression vector PJW4303. ELISA was used to detect the anti - Ag85B, MPT83 and MPT64 antibody titere in sera of the vaccinated cow and the concentration of IFN - ywas determined quantitatively with double antibodies sandwich ELISA. Specific antibody titere were negative with the preimmunization, which was increased slowly after the first and second injection and increased rapidly after third injection. MPT83 induced autibody titers were 1:3200 and kept for six months; Ag85B induced autibody titere were 1:3200 and kept for two months; MPT64 induced autibody titers were low. Antigen - specific IFN - ylevel of MPT83, Ag85B, MPT64 and PBS were (1 098.95 ± 165.03) pg/mL and (711.45 ± 110.43) pg/mL and (355.06 ±72.76) pg/mL and(114.5 ± 27.46) pg/mL after the sixth month of the third injection. The negative control group (PBS) showed very significant difference ( P < 0.01) with the three antigen groups. In conclusion, the triplevalent DNA vaccines stimulated cell - mediated immune responses as well as humoral responses. Our data suggest that polyvalent DNA vaccines are able to enhance protective response against M. TB.关键词
结核分枝杆菌/Ag85B/MPB64/MPT83/DNA疫苗/免疫应答Key words
Mycobacterium tuberculosis/Ag85B/MPB64/MPT83/DNA vaccine/immune response分类
农业科技引用本文复制引用
张云峰,刘福元,蔡宏,呼西旦,徐雪萍..结核分枝杆菌三价DNA疫苗在奶牛体内诱导的免疫应答[J].新疆农业科学,2011,48(7):1317-1323,7.基金项目
科技支疆项目(2008ZJ06) （2008ZJ06）
新疆农垦科学院青年基金(YQJ2009-04) （YQJ2009-04）
